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71.
Cibele de Cássia-Silva Marcus V. Cianciaruso Leandro Maracahipes Rosane G. Collevatti 《Plant Ecology》2017,218(10):1221-1231
Variations in abiotic characteristics such as soil water availability and fertility impose different selective pressures on plant populations. This may produce intraspecific variability in functional traits, even at a fine spatial scale. We investigated whether functional traits related to water-use efficiency, resource-retention strategy, soil nutrient acquisition, and fire tolerance differ in species that occur in two different habitats of Brazilian Cerrado: rocky savannas and savanna woodlands. Rocky savannas occur over sandstone, quartzite outcrops and have shallow nutrient-poor and low-moisture rocky soils, while savanna woodlands occur over well-drained and deep soils with frequent fire regimes. We measured nine functional traits of 40 tree species that occur in both habitats. Rocky savanna individuals exhibited a greater water-use efficiency strategy. The resource-retention strategy in rocky savanna individuals was corroborated by lower adult maximum height. However, despite the lower nutrient availability in rocky savanna soils, we only detected lower leaf phosphorus content in individuals from this habitat. Furthermore, individuals from both habitats had equally thick bark, suggesting that the fire-defense strategy is related to a stable, rather than plastic trait. Overall, our results highlight the central role of contrasting soil water availability patterns in driving phenotypic plasticity within species. We conclude that savanna species are responding to water and nutrient availabilities, via plasticity in traits related to the resource-retention strategy, and preparing for future fires, via uniformly thick bark. Wide plant distribution in contrasting habitats is possible for species that can shift ecological strategies to survive in nutrient- and water-limited habitats such as rocky savannas. 相似文献
72.
Craig B. H. Surman David W. Goodman 《Attention deficit and hyperactivity disorders》2017,9(3):161-168
ADHD is a neurodevelopmental syndrome that often persists into adulthood. It is possible that different criteria are necessary for older adults than younger adults: the manifestations of ADHD could change with age; other conditions with onset in later life share presenting symptoms with ADHD; different contextual challenges and patterns of compensatory support may exist. For these reasons, we reviewed evidence for the validity of DSM ADHD criteria in adulthood for individuals over the age of 50. Specifically, we evaluated evidence that the DSM criteria for ADHD identify a valid syndrome in older adults based on clinical presentation, laboratory or testing findings, absence of alternate diagnosis to explain symptoms, course of the syndrome, or familial presence of the condition. We found evidence that various ADHD criteria identify subjects with clinical presentations similar to that seen in younger adults, but only 92 well-described cases have been reported in the literature. ADHD traits also may be less common in the general population of older adults than in younger adults, suggesting that the threshold for an atypical burden of ADHD traits may be lower in older populations. Future research can establish a richer basis for validity of diagnostic criteria for ADHD in older adults. 相似文献
73.
Ratchada Cressey Saranya Pimpa Busyamas Chewaskulyong Nirush Lertprasertsuke Somchareon Saeteng Chatchai Tayapiwatana Watchara Kasinrerk 《BMC biotechnology》2008,8(1):16
Background
The recognition that human tumors stimulate the production of autoantibodies has initiated the use of this immune response as serological markers for the early diagnosis and management of cancer. The enzyme-linked immunosorbent assay (ELISA) is the most common method used in detecting autoantibodies, which involves coating the microtiter plate with the tumor associated antigen (TAA) of interest and allowing serum antibodies to bind. The patient's sample is directly in contact with the coating antigen so the protein used for coating must be pure to avoid non-specific binding. In this study, a simplified method to selectively and specifically immobilize TAAs onto microtiter plates in order to detect circulating autoantibodies in cancer patients without prior purification process was described. Wild type full-length p53 protein was produced in fusion with biotin carboxyl carrier peptide (BCCP) or hexahistidine [(His)6] using pAK400 and pET15b(+) vectors, respectively. The recombinant p53 fusion protein produced was then subjected to react with either a commercial p53 monoclonal antibody (mAb) or sera from lung cancer patients and healthy volunteers in an enzyme-linked immunosorbent assay (ELISA) format. 相似文献74.
Light is known to regulate conservative germination strategies and the formation of seed banks. Although these strategies
are crucial to survival in tundra environments—especially for annuals—light requirements for germination in arctic–alpine
species are seldom investigated. Furthermore, environmental differences between arctic and alpine regions are expected to
lead to evolutionary divergence among conspecific populations in seed germination strategies. In this study, we report important
differences in germination light requirements among six arctic and alpine populations of the annual Koenigia islandica. Light had little effect on germination of the seeds from Iqaluit (Nunavut, Canada), Yukon (Canada), and Jasper (Alberta,
Canada), whereas the seeds from the most severe climates, Svalbard (Norway) and Colorado (USA), had strong light requirements.
Stratification of the seeds had little influence on their germination light requirements, with the exception of the population
from Dovre (Norway), in which it induced a strong light requirement. Possible adaptive explanations and some implications
of these observed germination patterns are discussed. 相似文献
75.
David H. Jewson Nick G. Granin Andre A. Zhdanov Ruslan Yu Gnatovsky 《Aquatic Ecology》2009,43(3):673-679
Lake Baikal freezes for 4–5 months each year; yet the planktonic diatoms that grow under the ice include some of the largest
species found in freshwater. An important factor influencing their growth is the depth of snow. In this study, a population
of Aulacoseira baicalensis developed where there was little or no snow on the ice but declined where there was 10 cm of snow, because 99% of the available
light was attenuated. Culture studies of light response showed that A. baicalensis was adapted to relatively low light intensities (<40 μmol m−2 s−1) that were close to the average that a cell experiences in L. Baikal when mixed vertically by convection to depths that can
exceed 100 m. On sunny days, cell division could be inhibited down to >10 m depth but narrow (<15 μm) diameter cells trapped
in high light intensities in sub-ice layers switched to auxosporulation and size regeneration. 相似文献
76.
77.
Marta Tejera-Alhambra Armanda Casrouge Clara de Andrés Ansgar Seyfferth Rocío Ramos-Medina Bárbara Alonso Janet Vega Lidia Fernández-Paredes Matthew L. Albert Silvia Sánchez-Ramón 《PloS one》2015,10(6)
Multiple sclerosis, the most common cause of neurological disability in young population after trauma, represents a significant public health burden. Current challenges associated with management of multiple sclerosis (MS) patients stem from the lack of biomarkers that might enable stratification of the different clinical forms of MS and thus prompt treatment for those patients with progressive MS, for whom there is currently no therapy available. In the present work we analyzed a set of thirty different plasma cytokines, chemokines and growth factors present in circulation of 129 MS patients with different clinical forms (relapsing remitting, secondary progressive and primary progressive MS) and 53 healthy controls, across two independent cohorts. The set of plasma analytes was quantified with Luminex xMAP technology and their predictive power regarding clinical outcome was evaluated both individually using ROC curves and in combination using logistic regression analysis. Our results from two independent cohorts of MS patients demonstrate that the divergent clinical and histology-based MS forms are associated with distinct profiles of circulating plasma protein biomarkers, with distinct signatures being composed of chemokines and growth/angiogenic factors. With this work, we propose that an evaluation of a set of 4 circulating biomarkers (HGF, Eotaxin/CCL11, EGF and MIP-1β/CCL4) in MS patients might serve as an effective tool in the diagnosis and more personalized therapeutic targeting of MS patients. 相似文献
78.
Matthew E. Downs Amanda Buch Carlos Sierra Maria Eleni Karakatsani Shangshang Chen Elisa E. Konofagou Vincent P. Ferrera 《PloS one》2015,10(5)
Focused Ultrasound (FUS) coupled with intravenous administration of microbubbles (MB) is a non-invasive technique that has been shown to reliably open (increase the permeability of) the blood-brain barrier (BBB) in multiple in vivo models including non-human primates (NHP). This procedure has shown promise for clinical and basic science applications, yet the safety and potential neurological effects of long term application in NHP requires further investigation under parameters shown to be efficacious in that species (500kHz, 200–400 kPa, 4–5μm MB, 2 minute sonication). In this study, we repeatedly opened the BBB in the caudate and putamen regions of the basal ganglia of 4 NHP using FUS with systemically-administered MB over 4–20 months. We assessed the safety of the FUS with MB procedure using MRI to detect edema or hemorrhaging in the brain. Contrast enhanced T1-weighted MRI sequences showed a 98% success rate for openings in the targeted regions. T2-weighted and SWI sequences indicated a lack edema in the majority of the cases. We investigated potential neurological effects of the FUS with MB procedure through quantitative cognitive testing of’ visual, cognitive, motivational, and motor function using a random dot motion task with reward magnitude bias presented on a touchpanel display. Reaction times during the task significantly increased on the day of the FUS with MB procedure. This increase returned to baseline within 4–5 days after the procedure. Visual motion discrimination thresholds were unaffected. Our results indicate FUS with MB can be a safe method for repeated opening of the BBB at the basal ganglia in NHP for up to 20 months without any long-term negative physiological or neurological effects with the parameters used. 相似文献
79.
Pernilla Christensen Frauke Ecke Per Sandström Mats Nilsson Birger Hörnfeldt 《Population Ecology》2008,50(2):169-179
There has been a long-term decline in spring and fall numbers of Clethrionomys rufocanus in boreal Sweden in 1971–2005. Previous studies on permanent sampling plots in the centre of 2.5 × 2.5 km landscapes suggested
that habitat fragmentation (sensu destruction) could have contributed to the decline. Therefore, we tested these findings
in a field study and compared trapping results on the central sampling plots of landscapes with a low degree of fragmentation
(LDF) and of “hot spot” type with trapping results in managed forest landscapes with a high degree of fragmentation (HDF).
We predicted that C. rufocanus would be more common on the LDF plots. We used our permanent plots supplemented with a new sample of plots, mainly of the
rare LDF type, inside or just outside the long-term study area. Very few voles were trapped on both plot types, and no difference
was found. However, a subsequent pilot study with trapping in a national park with large areas of pristine, unfragmented forest
yielded more voles than in the managed, more fragmented, areas. Consequently, the initial field study data and some other
recent data were also re-analysed from a “local patch quality” perspective. This alternative approach revealed the positive
importance of large focal patches of forest >60 years old and their content of old-growth (pine) forest (>100 years). Interestingly,
at the landscape level, the frequency distribution of patches of forest >60 years old, old-growth (>100 years), and especially
of old-growth pine forest (>100 years), relative to the properties of plots with C. rufocanus, suggested that there are few forest patches left that are suitable for C. rufocanus. Our current results suggest that habitat fragmentation cannot be excluded as a contributing cause to the long-term decline
of C. rufocanus in boreal Sweden. 相似文献
80.